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September 02, 2015 9:35 AM ET

Life Sciences Tools and Services

Company Overview of Promega Corporation

Company Overview

Promega Corporation provides solutions and technical support services for the life sciences industry. It manufactures reagents and instrumentations; and offers products in the areas of genomics, protein analysis and expression, cellular analysis, drug discovery, and genetic identity. The company also provides DNA and RNA quantitation, DNA extraction, and sample ID and mixed sample detection products; and bio chemicals and laboratory ware, such as buffers and reagents, nucleic acids, and nucleic acids purification accessories. In addition, it offers products and services in the areas of biologics, cell health and metabolism, cell signaling, cloning and DNA markers, DNA and RNA purification, d...

2800 Woods Hollow Road

Madison, WI 53711

United States

Founded in 1978





Key Executives for Promega Corporation

Co-Founder, Chairman and Chief Executive Officer
Age: 67
Chief Technical Officer and Vice President
Senior Executive Officer
Age: 63
Vice President of Global Sales
Age: 55
Compensation as of Fiscal Year 2015.

Promega Corporation Key Developments

Promega Corporation Announces Launch of NanoBRET Protein Interaction Assays

Promega Corporation announced the launch of NanoBRET Protein Interaction Assays, which use a new Bioluminescence Resonance Energy Transfer (BRET) technology that enables scientists to quantitatively measure protein: protein interactions in live cells. Traditional methods for studying interactions between proteins are commonly performed in vitro using only protein fragments and do not provide data in the context of the cellular environment. With NanoBRET Protein Interaction Assays, researchers can study both induction and inhibition of protein interactions in real time using full-length proteins expressed at physiologically relevant levels. Conventional BRET measures the interaction of proteins using a bioluminescent donor fused to a protein of interest and a fluorescent acceptor fused to its binding partner; the donor does not excite the fluorophore using light, but transfers resonance energy through dipole-dipole coupling. The optimized NanoBRET Protein Interaction Assays use NanoLuc® Luciferase as the energy donor and HaloTag® protein as the energy acceptor. NanoBRET Technology has improved spectral overlap, increased signal, and lower background, providing researchers with a reproducible method for monitoring and screening protein interactions. In addition, the brighter light output from NanoLuc enables use of NanoBRET even at low expression levels, while still providing efficient energy transfer.

Promega Corporation Announces Launch of GloMax Explorer Multimode Detection System, a Flexible Multimode Reader for Luminescence, Fluorescence and Absorbance Detection

Promega Corporation announced the launch of GloMax Explorer Multimode Detection System, a flexible multimode reader for luminescence, fluorescence and absorbance detection integrated with over 50 optimized, preloaded Promega protocols to address a variety of research needs. The GloMax Explorer System is designed with preloaded Promega protocols to minimize time spent optimizing instrument settings. Complemented by the wide range of integrated Promega assays, including nucleic acid quantitation for next-generation sequencing, GloMax Explorer provides high sensitivity, a broad dynamic range and low well-to-well cross talk that generate more usable and relevant data. Users also have the option of customizing their own protocols in accordance with specific needs. As the newest member of the GloMax® System detection instruments, GloMax Explorer allows researchers to choose a range of detection modules for their current work and upgrade capabilities as their experimental needs grow. Multiplexed cell viability, apoptosis and cytotoxicity assays are easily performed with minimal manual intervention because of automated filter switching. The software is SiLA compatible, making integration of device control simple. Using the instrument’s touchscreen display and automatic gain adjustments, researchers can quickly generate and analyze data from critical experiments with minimal set-up time and effort. From the touchscreen, users can set up a plate map, view data in a heat map display and easily export their results to a variety of locations without complicated adjustments.

Promega Corporation Launches Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay

Promega Corporation announced the launch of the new Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay, a two-reporter system that incorporates NanoLuc luciferase technology, providing increased data quality and greater sensitivity for biologically complex applications. The new NanoDLR assay allows researchers to measure NanoLuc and firefly luciferases together in a convenient, easy-to-use format. With improved firefly chemistry and the small, ultra-sensitive NanoLuc luciferase, researchers gain more sensitivity to detect small changes in expression, more flexibility in assay design and more robust control reporter options. In the NanoDLR Assay, firefly luciferase, NanoLuc luciferase, or both, can be used as dynamic reporters, greatly increasing versatility by allowing researchers to choose the primary reporter that best meets their experimental needs. The new assay chemistry provides improved reagent stability over time, giving more consistent results and eliminating the need to freeze reagent. NanoDLR is a homogenous reagent system with stable glow-type luminescent signals, but can also be used with injection-based protocols or cell lysates, and is compatible with automated liquid handling and high density plates, including 1536-well formats. One novel application of the two powerful primary reporters in the NanoDLR assay is the coincidence reporter assay, an innovation with great promise for high-throughput chemical library screening in drug discovery. This type of assay uses two independent reporters to measure a single transcription event, providing a way to distinguish true “hits” from experimental artifacts caused by interaction between library compounds and reporter proteins. Along with the release of the NanoDLR Assay, several new NanoLuc and firefly luciferase plasmids are available. These include control vectors with the option of using the native phosphoglycerate kinase (PGK) promoter to replace promoters of viral origin, and plasmids for coincident reporter assays for high-throughput chemical library screening.

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