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May 05, 2015 7:02 AM ET

Life Sciences Tools and Services

Company Overview of Promega Corporation

Company Overview

Promega Corporation provides solutions and technical support services for the life sciences industry. It manufactures reagents and instrumentations; and offers products in the areas of genomics, protein analysis and expression, cellular analysis, drug discovery, and genetic identity. The company also provides DNA and RNA quantitation, DNA extraction, and sample ID and mixed sample detection products; and bio chemicals and laboratory ware, such as buffers and reagents, nucleic acids, and nucleic acids purification accessories. In addition, it offers products and services in the areas of biologics, cell health and metabolism, cell signaling, cloning and DNA markers, DNA and RNA purification, d...

2800 Woods Hollow Road

Madison, WI 53711

United States

Founded in 1978

Phone:

608-274-4330

Fax:

608-277-2516

Key Executives for Promega Corporation

Co-Founder, Chairman and Chief Executive Officer
Age: 66
Vice President of Finance and Chief Financial Officer
Age: 47
Chief Technical Officer and Vice President
Senior Executive Officer
Age: 62
Vice President of Global Sales
Compensation as of Fiscal Year 2014.

Promega Corporation Key Developments

Promega Corporation Launches Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay

Promega Corporation announced the launch of the new Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay, a two-reporter system that incorporates NanoLuc luciferase technology, providing increased data quality and greater sensitivity for biologically complex applications. The new NanoDLR assay allows researchers to measure NanoLuc and firefly luciferases together in a convenient, easy-to-use format. With improved firefly chemistry and the small, ultra-sensitive NanoLuc luciferase, researchers gain more sensitivity to detect small changes in expression, more flexibility in assay design and more robust control reporter options. In the NanoDLR Assay, firefly luciferase, NanoLuc luciferase, or both, can be used as dynamic reporters, greatly increasing versatility by allowing researchers to choose the primary reporter that best meets their experimental needs. The new assay chemistry provides improved reagent stability over time, giving more consistent results and eliminating the need to freeze reagent. NanoDLR is a homogenous reagent system with stable glow-type luminescent signals, but can also be used with injection-based protocols or cell lysates, and is compatible with automated liquid handling and high density plates, including 1536-well formats. One novel application of the two powerful primary reporters in the NanoDLR assay is the coincidence reporter assay, an innovation with great promise for high-throughput chemical library screening in drug discovery. This type of assay uses two independent reporters to measure a single transcription event, providing a way to distinguish true “hits” from experimental artifacts caused by interaction between library compounds and reporter proteins. Along with the release of the NanoDLR Assay, several new NanoLuc and firefly luciferase plasmids are available. These include control vectors with the option of using the native phosphoglycerate kinase (PGK) promoter to replace promoters of viral origin, and plasmids for coincident reporter assays for high-throughput chemical library screening.

Promega Corporation Releases PowerQuant™ System

Promega Corporation announced the release of the PowerQuant™ System, a probe-based 4-target, 5-dye real-time PCR method. The system allows laboratories to determine human and male DNA concentrations in a sample, assess the integrity of the DNA sample and detect possible PCR inhibitors in approximately one hour. The PowerQuant System ensures that DNA detected in the quantification step of the workflow equates to a usable STR profile. Accurate and sensitive DNA quantification plays a key role in ensuring successful STR results, and because many samples are precious, getting it right the first time is critical. Inaccurate determination of the amount of DNA present in the sample can lead to unusable STR profiles, and repeating the STR assay with corrected DNA template amounts can waste both reagents and a user’s time. PowerQuant also allows laboratories to assess the quality of the DNA sample, whether it is the integrity of the DNA or the presence of possible PCR inhibitors. By correctly doing so, laboratories can make informed decisions about processing difficult casework samples. Using the PowerQuant System, along with the PowerQuant™ Analysis Tool program, for quality assessment and STR normalization values, laboratories are able to make the best decision about what steps to take next in their workflow and eliminate guesswork about DNA samples.

Promega Corporation Announces the Release of 6 × 5 LC-MS/MS Peptide Reference Mix

Promega Corporation announced the release of the 6 × 5 LC-MS/MS Peptide Reference Mix, a novel reagent and software combination. The reagent monitors LC and MS parameters, while the complementary software reports on instrument performance, as well as performance history. The unique peptide formulation saves time by assessing multiple LC and MS parameters, such as peak height, mass accuracy, dynamic range and sensitivity, in a single run. The AAA-qualified peptides result in accurate reporting of instrument sensitivity and dynamic range. The 6 × 5 LC-MS/MS Peptide Reference Mix can be used in multiple applications, with either neat or complex sample mixtures. The complementary PReMiS Software tool eliminates time-consuming manual calculations, and monitors instrument-to-instrument performance over time. Hardware required to run the PReMiS Software includes a Windows® PC with a Dual-Core x86 based processor, 4GB Memory, 100GB hard drive, video 1024 × 768 pixels, and Microsoft Windows® XP® SP3, Vista® 32/64, Windows® 7 32/64, or Windows® 8 32/64.

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