Ashton was suspended for last week’s 54-12 win over Fiji and takes over on right wing for Charlie Sharples, who switches to the left side in place of Ugo Monye. It’s the only change made by coach Stuart Lancaster.
Australia made four changes from the team which lost 33-6 to France last week in Paris.
Berrick Barnes replaces Mike Harris at fullback, Digby Ioane takes over for Pat McCabe on right wing, Sitaleki Timani moves into the second in place of Kane Douglas and Ben Alexander starts at tighthead prop ahead of Sekope Kapu.
England team: Alex Goode (Saracens), Chris Ashton (Saracens), Manu Tuilagi (Leicester), Brad Barritt (Saracens), Charlie Sharples (Gloucester), Toby Flood (Leicester), Danny Care (Harlequins), Joe Marler (Harlequins), Tom Youngs (Leicester), Dan Cole (Leicester), Tom Palmer (Wasps), Geoff Parling (Leicester), Tom Johnson (Exeter), Chris Robshaw (Harlequins, captain), Thomas Waldrom (Leicester). Replacements: David Paice (London Irish), David Wilson (Bath), Mako Vunipola (Saracens), Joe Launchbury (Wasps), Tom Wood (Northampton), Ben Youngs (Leicester), Owen Farrell (Saracens), Mike Brown (Harlequins). Australia team: Berrick Barnes (NSW Waratahs), Digby Ioane (Queensland Reds), Adam Ashley-Cooper (NSW Waratahs), Ben Tapuai (Queensland Reds), Nick Cummins (Western Force), Kurtley Beale (Melbourne Rebels), Nick Phipps (Melbourne Rebels), Benn Robinson (NSW Waratahs), Tatafu Polota Nau (NSW Waratahs), Ben Alexander (Brumbies), Sitaleki Timani (NSW Waratahs), Nathan Sharpe (Western Force, captain), Dave Dennis (NSW Waratahs), Michael Hooper (NSW Waratahs), Wycliff Palu (NSW Waratahs). Replacements: Stephan Moore (Brumbies), James Slipper (Queensland Reds), Sekope Kepu (NSW Waratahs), Radike Samo (Queensland Reds), Liam Gill (Queensland Reds), Brett Sheehan (Western Force), Mike Harris (Queensland Reds), Drew Mitchell (NSW Waratahs).