NEB Releases a New Site-Directed Mutagenesis Kit for Both Standard and Novel Uses, Including Long Insertions and Deletions

 NEB Releases a New Site-Directed Mutagenesis Kit for Both Standard and Novel
                Uses, Including Long Insertions and Deletions

Includes Unique Features for Difficult-to-Amplify GC-Rich and Repetitive

PR Newswire

IPSWICH, Mass., May 10, 2013

IPSWICH, Mass., May 10, 2013 /PRNewswire/ -- New England Biolabs (NEB), a
world leader in the discovery and production of reagents for the life science
industry, launched a new reagent kit for site-directed mutagenesis (SDM). The
Q5^® Site-Directed Mutagenesis Kit (Q5 SDM Kit) is capable of introducing long
insertions and deletions, thus allowing a broader range of applications for
this widely-used laboratory technique. The Q5 SDM Kit can make standard
single-base modifications, as well as novel manipulations, such as deletions
or additions of any length, including the addition of nuclear localization
signals to cloned proteins.

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"The Q5 SDM Kit makes applications possible for which you might not have
thought of using SDM. For example, SDM can be a much quicker and less
error-prone way to add tags, make large deletions, add or remove restriction
sites, and many other sequence modifications," says John Pezza, Ph.D., an
Applications and Product Development Scientist at NEB.

NEB adopted a non-overlapping primer design to allow for longer modifications.
The most popular SDM methods use overlapping primers that limit the length of
insertions or deletions. NEB's approach enables exponential amplification and
more efficient plasmid transformation, which facilitates the successful
modification of highly repetitive or GC-rich sequences.

NEB has also released an SDM primer design tool, NEBaseChanger™, which
supports the Q5 SDM Kit. Developed by Sanjay Kumar, Ph.D., Senior Research
Scientist at NEB, the new web tool includes a novel feature, the application
of nearest neighbor thermodynamics for matched and mismatched bases to design
mutational SDM primers and calculate custom annealing temperatures. "These
adjustments increase the odds that the experiment will work. Most, if not all
other design tools, are not able to take mismatches into account," says Pezza.
NEBaseChanger also provides live primer design updates, so users can make
iterative changes to their primers and watch how details like reading frame
and Tm are affected.

The Q5 SDM Kit includes Q5 Hot Start High-Fidelity Polymerase, which has been
tested to amplify sequences with a fidelity 100-times greater than Taq and
twice that of Phusion^® DNA Polymerase. The kit also includes high-efficiency
NEB 5-alpha Competent E. coli. Robust results have been observed with plasmids
up to, at least, 14.3 kb.

To learn more, visit

About New England Biolabs
Established in the mid 1970's, New England Biolabs, Inc. is the industry
leader in the discovery and production of enzymes for molecular biology
applications and now offers the largest selection of recombinant and native
enzymes for genomic research. NEB continues to expand its product offerings
into areas related to PCR, gene expression, library preparation for next
generation sequencing, cellular analysis, epigenetics and RNA analysis.
Additionally, NEB is focused on strengthening alliances that enable new
technologies to reach key market sectors. New England Biolabs is a privately
held company, headquartered in Ipswich, MA, and has extensive worldwide
distribution through a network of exclusive distributors, agents and seven
subsidiaries located in Canada, China, France, Germany, Japan, Singapore and
the UK. For more information about New England Biolabs

NEW ENGLAND BIOLABS^® and Q5^® are registered trademarks of New England
Biolabs, Inc.
NEBASECHANGER™ is a trademark of New England Biolabs, Inc.
PHUSION^® is a registered trademark of Thermo Fisher Scientific.

SOURCE New England Biolabs

Contact: Deana D. Martin, Ph.D., Marketing Communications Manager, New England
Biolabs, 978-380-7464,
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