Pacific Biosciences' Software Upgrade Enhances De Novo Genome Assembly, Variant Calling and cDNA Transcript Analysis

Pacific Biosciences' Software Upgrade Enhances De Novo Genome Assembly,
Variant Calling and cDNA Transcript Analysis

MENLO PARK, Calif., Jan. 29, 2013 (GLOBE NEWSWIRE) -- Pacific Biosciences of
California, Inc. (Nasdaq:PACB) provider of the PacBio^® RS High Resolution
Genetic Analyzer, today announced it is releasing a new software upgrade that
provides higher quality genome assemblies with near perfect base level
accuracy in addition to other key features. The software upgrade extends the
range of projects that uniquely benefit from the company's Single Molecule,
Real-Time (SMRT^®) DNA Sequencing method, and will be available to customers
for download on January 31^st.

SMRT Analysis 1.4 includes a new hierarchical de novo genome assembly process
(HGAP), which allows researchers to assemble entire microbial and fungal
genomes solely using PacBio long reads. As a result, users can generate better
assemblies with a single library preparation and fewer SMRT^® Cells than
previous approaches that also required short-read sequencing technologies or
circular consensus sequencing.

SMRT Analysis 1.4 also incorporates a new multi-read consensus algorithm
called Quiver to determine the finished genome sequence with exceptional
accuracy. Quiver can provide greater than 99.999% consensus accuracy for both
resequencing and de novo assembly applications.

"The extra-long sequence reads and the subsequenthighly accuratede
novoassemblies produced by the PacBio RS through the latest software
enhancements have immensely improved our ability to discover and understand
novel genomes cost-effectively," said J. Craig Venter, Ph.D., Founder,
Chairman, and Chief Executive Officer at The J. Craig Venter Institute and CEO
of Synthetic Genomics.

The software upgrade also includes support for analyzing full-length cDNA
transcripts, facilitating understanding of transcription, gene structure and
alternative splicing. Unlike any other sequencing technology, SMRT Sequencing
can span entire cDNA transcripts with a single read, revealing the complete
exonic structure of transcripts.

Additional features of the new SMRT Analysis 1.4 upgrade include:

  *Support for analysis of barcoded samples;
  *Improvements to the tools for analyzing and visualizing bacterial
    methylomes, a capability unique to SMRT Sequencing;
  *Enhanced user and group permissions to help sequencing centers and core
    labs control what data is presented to end users.

"We are committed to continually improving the performance of the PacBio RS,
in part through developing software enhancements designed to leverage the
unique characteristics of our system," said Kevin Corcoran, Senior Vice
President of Market Development at Pacific Biosciences. "With long sequence
reads, gold standard accuracy and higher quality assemblies, the genomics
community can enjoy the benefits of truly finished genomes through SMRT

The new SMRT Analysis software upgrade is available for download from Pacific
Biosciences' community website, along with sample data and protocols for cDNA
sample preparation and barcoding. To access the software, data, and
documentation, visit

For more information on the new SMRT Analysis software and the PacBio RS High
Resolution Genetic Analyzer, please visit 

About Pacific Biosciences

Pacific Biosciences of California, Inc. (Nasdaq:PACB) offers the PacBio^® RS
High Resolution Genetic Analyzer to help scientists solve genetically complex
problems. Based on its novel Single Molecule, Real-Time (SMRT^®) technology,
the company's products enable: targeted sequencing to more comprehensively
characterize genetic variations; de novo genome assembly to more fully
identify, annotate and decipher genomic structures; and DNA base modification
identification to help characterize epigenetic regulation and DNA damage. By
providing access to information that was previously inaccessible, Pacific
Biosciences enables scientists to increase their understanding of biological

         Maurissa Messier
         For Pacific Biosciences
         Trevin Rard
         Pacific Biosciences
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