New England Biolabs Releases a New Fast, Low-Nanogram Input Library Prep Method for DNA and RNA Sequencing

   New England Biolabs Releases a New Fast, Low-Nanogram Input Library Prep
                      Method for DNA and RNA Sequencing

PR Newswire

IPSWICH, Mass., Nov. 16, 2012

IPSWICH, Mass., Nov. 16, 2012 /PRNewswire/ --New England Biolabs, Inc. (NEB)
launched their new NEBNext^® "Ultra" kits at the recent American Society for
Human Genetics (ASHG) annual meeting. These kits provide streamlined,
low-input methods to prepare DNA and RNA libraries for Illumina^® next
generation sequencing. The supplied protocols and reagents are designed to
maximize useful data from a broad range of samples, including those available
in limited amounts.

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The NEBNext Ultra DNA and Ultra RNA Library Kits produce high-yield libraries
from 5 ng to 1 ug of input DNA, or as little as 10 ng of input RNA. The input
RNA can be total RNA, purified mRNA or rRNA-depleted RNA.

Early access user Cynthia Hendrickson, Ph.D., at the HudsonAlpha Institute for
Biotechnology, reports that in initial experiments, NEB's new method "has
allowed us to reduce our DNA inputs for exome enrichment from micrograms to a
few hundred nanograms, while providing a simpler, faster protocol that
minimizes the amount of hands-on time required."

Another early access user, Momchilo Vuyisich, Ph.D., at the Los Alamos
National Laboratory, says, "After testing several NGS library prep kits, we
have found that the NEBNext Ultra DNA kit for Illumina has the best overall
utility for resequencing and assembly of bacterial genomes. The kit offers an
unmatched combination of ease-of-use, low cost, robustness and low input DNA
requirements. It consistently produces excellent sequencing data."

The new Ultra kits contain novel ligation reagents, as well as NEB's NEBNext
NGS-optimized formulation of Q5^® High-Fidelity DNA polymerase, which provides
ultra high-fidelity amplification and minimized GC bias.

NEB's streamlined protocol for constructing DNA libraries with the Ultra kit
requires only 15 minutes of hands-on-time, and is complete in 2.5 hours. The
Ultra RNA workflow also incorporates a streamlined protocol; the workflow is
complete in 4-5 hours, with only 30 minutes of hands-on time.

For more information on NEB's new Ultra DNA and RNA kits, visit 

About New England Biolabs
Established in the mid 1970's, New England Biolabs, Inc. is the industry
leader in the discovery and production of enzymes for molecular biology
applications and now offers the largest selection of recombinant and native
enzymes for genomic research. NEB continues to expand its product offerings
into areas related to PCR, gene expression, sample preparation for next
generation sequencing, cellular analysis, epigenetics and RNA analysis.
Additionally, NEB is focused on strengthening alliances that enable new
technologies to reach key market sectors. New England Biolabs is a privately
held company, headquartered in Ipswich, MA, and has extensive worldwide
distribution through a network of exclusive distributors, agents and six
subsidiaries located in Canada, China, France, Germany, Japan and the UK. For
more information about New England Biolabs visit

ILLUMINA^® is a registered trademark of Illumina, Inc.
NEBNEXT^® and Q5^® are registered trademarks of New England Biolabs, Inc.

SOURCE New England Biolabs, Inc.

Contact: Deana D. Martin, Ph.D., Marketing Communications Manager, New England
Biolabs, +1-978-380-7464,
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